2型糖尿病和冠心病患者血浆促酰化蛋白水平的研究

Study of Plasma Acylation-Stimulating Protein in Diabetic and Coronary Heart Disease Patients

目的 :测定 2型糖尿病和冠心病患者血浆促酰化蛋白 (ASP)水平 ,探讨血浆ASP与体重指数、血脂的相关关系。方法 :实验分 3组 :正常对照组 47例、糖尿病组 63例、冠心病组 62例 ,记录每例被试对象的年龄、性别、身高、体重、血压 ,并计算体重指数 (BMI)。酶联免疫吸附分析法 (ELISA)检测血浆ASP浓度 ,免疫比浊法检测血脂等生化指标。结果 :①糖尿病组、冠心病组ASP水平明显高于正常对照组 (P <0 0 5 )。②ASP与体重指数、收缩压、舒张压、总胆固醇、甘油三酯、低密度脂蛋白胆固醇、载脂蛋白B10 0呈显著正相关 (分别为r =0 3 2 ,P <0 0 1;r =0 2 4,P <0 0 1;r =0 19,P <0 0 1;r =0 3 5 ,P <0 0 1;r =0 10 ,P <0 0 5 ;r =0 2 9,P <0 0 1;r =0 3 2 ,P <0 0 1)。③多元逐步回归分析 :载脂蛋白B10 0、体重指数、高密度脂蛋白胆固醇和舒张压最终进入方程 ,它们是影响血浆ASP水平的重要因素 ,其中载脂蛋白B10 0的作用最大。结论 :ASP参与了糖尿病和冠心病患者脂质代谢紊乱的发生 。

Objective:To estimate the plasma acylation-stimulating protein(ASP)level in diabetic and coronary heart disease patients,and to investigate the correlation of plasma ASP with body mass index(BMI),low density lipoprotein cholesterol:(LDL-C),high density lipoprotein cholesterol(HDL-C),total cholesterol(TC),triglyceride(TG)and apolipoprotein. Methods:Oen hundred Seventy-two subjects were enrolled in this study,including 63 diabetics,62 coronary heart disease patients and 47 health controls.ELISA technique was employed to determine the plasma ASP concentration. Results:① The levels of plasma ASP were increased significantly in diabetes group and coronary heart disease group compared with the controlgroup(p<0.05).② Plasma ASP was positively correlated with BMI,systolic pressure,diastolic pressure,total cholesterol,triglyceride,LDL-c and ApoB100(r=0.32,p<0.01;r=0.24,p<0.01;r= 0.19,p<0.01;r=0.35,p<0.01;r=0.10,p<0.05;r=0.29,p<0.01;r=0.32,p<0.01 respectively).③Multiple regression analysis was performed for BMI,systolic pressure,TC,TG,HDL-C,LDL-C,ApoB100,and diabetes and coronary heart disease.Factor such as ApoB100,BMI,LDL-C and diastolic pressure exerted an important effect on plasma ASP level,especially ApoB100. Conclusion:Plasma ASP is involved in the development of the disordered lipid metabolism in diabetes and coronary heart disease.ASP can be used as a new indicator for estimating insulin resistance in diabetics.Plasma ASP provides us a new index in predicting the cardiovascular risk for diabetic patients.