补阳还五汤对沙土鼠脑缺血再灌注损伤后血清炎性细胞因子IL-1β、IL-6、TNF-α的影响

The Effects of Bu-Yang-Huan-Wu Decoction on Inflammatory Cellular Factors in Blood Serums after the Injury of Cerebral Ischemia and Reperfusion in Gerbils

目的:探讨补阳还五汤对脑缺血再灌注损伤后炎性细胞因子IL-1β、IL-6、TNF-α蛋白表达的影响。方法:采用Kirino蒙古沙土鼠双侧颈总动脉夹闭脑缺血模型,缺血15min后进行再灌注;于再灌注48h后,测定血清IL-1β、IL-6、TNF-α的含量。结果:补阳还五汤组的IL-1β、IL-6、TNF-α均含量明显低于模型组(P <0.01)和标准对照组(P <0.01),但补阳还五汤组和标准对照组均显著高于假手术组(P <0.01)。结论:补阳还五汤可拮抗脑缺血再灌注损伤后的炎性细胞因子,因而减轻脑缺血再灌注引起的损伤。

To investigate the effects of Bu Yang Huan Wu Decoction (BYHWD)on inflammatory cellular factors. Methods: Cerebral ischemic reperfusion model in gerbils was used. Bilateral carotid arteries were ligated for 15 minutes, then reperfused for 48 hours. The contents of IL-1β, IL-6 and TNF-αin blood serums were detected and compared in different groups. Result: In comparison with ischemia-reperfusion (IR)and standardized-comparison(SC)group, IL-1β?IL-6 and TNF-αwere significantly decreased in BYHWD group(all P <0.01).Compared with sham-operation(SO)group, IL-1β?IL-6 and TNF-αin BYHWD and SC group were significantly increased (P <0.01).Conclusion:BYHWD has a protective effect on nervous injury in cerebral ischemia and reperfusion by reduction of inflammatory cellular factors.