肠道螺旋体对肠上皮细胞的吸附及其特性的研究

Attachment of Brachyspira pilosicoli to intestinal epithelial cells

目的 研究肠道螺旋体对肠上皮细胞吸附的特性。方法 利用人工培养的肠上皮细胞系H40 7细胞建立研究肠道螺旋体与肠上皮细胞相互作用的方法 ,并用该方法研究肠道螺旋体对肠上皮细胞吸附的特性。结果 4种不同来源的肠道螺旋体分离株均能吸附到人肠上皮细胞系H40 7细胞的表面 ,其中以人的分离株SP16的吸附率为最高 ,而非致病性的BrachyspirainnocensB2 5 6株则没有明显的吸附。人分离株SP16与H40 7细胞共同孵育 1h后的吸附率为 63 .77% ,2h则达到 89.11%。H40 7细胞经固定后肠道螺旋体仍能有效地吸附到细胞上 ,显示受体是稳定表达在肠上皮细胞的表面。结论 利用人工培养的肠上皮细胞系H40 7细胞建立了研究肠道螺旋体与肠上皮细胞相互作用的体外模型。

Objective To characterize the attachment of B. pilosicoli to intestinal epithelial cells. Methods Cultured human intestinal epithelial cell line H407 was employed for the establishment of an in vitro attachment assay for the study of the mechanism of attachment of B. pilosicoli to intestinal epithelial cells. Results Four strains of B. pilosicoli isolated from human and animals could attach to H407 intestinal epithelial cells, and the human isolate SP16 had the highest attachment rate. The attachment rate of SP16 reached 63.77% at 1 h and 89.11% at 2 h after coincubation of SP16 with H407 cells. When H407 cells were fixed, human isolate SP16 could still attach to the cells efficiently, suggesting stable expression of receptor on the cell surface. Conclusion An in vitro attachment assay using human intestinal epithelial cell line H407 has been established and can be used to study of interactions of B. pilosicoli with human intestinal epithelial cells in vitro.