茶树油灭活脊髓灰质炎病毒的试验观察

Experimental observation of the inactivating effect of tea tree oil on poliovirus

目的 观察茶树油 (teatreeoil,TTO)对脊髓灰质炎病毒 (poliovirus ,PV)的杀灭效果 ,了解TTO对病毒的灭活能力。方法 根据消毒技术规范指定的方法 ,采用细胞感染试验测定病毒与各浓度TTO溶液作用前后感染细胞的滴度。结果 0 .5 %TTO作用 40min和 60min能分别灭活PV 4.3 3log10和 5 .5log10 ;1.0 %TTO作用 40min和 60min能灭活PV 5 .3 3log10和 5 .5log10。结论 TTO对悬液中的PV有明显的杀灭作用。

Objective To evaluate the virucidal efficacy of tea tree oil (TTO) on poliovirus (PV). Methods According to the Technical Standard For Disinfection, the PV-inactivating capacity of TTO was objectively detected by cell infection method before and after treatment of the virus with different concentrations of TTO. Results A 4.33 log10 titre reduction and a 5.5 log10 titre reduction of PV could be obtained with 0.5% TTO for 40 and 60 min, respectively; and a 5.33 log10 titre reduction and a 5.5 log10 titre reduction could be obtained with 1.0% TTO for 40 and 60 min, respectively. Conclusion TTO has obvious inactivating effect on PV in vitro.