Stat1蛋白在急性髓系细胞白血病患者外周血白细胞中的激活

Activation of the Stat1Protein in the Peripheral Blood White Cells of the Patients with Acute Myeloid Leukemia

背景与目的 :了解急性髓系细胞白血病外周血白细胞Stat1蛋白表达情况 ,探讨Stat1蛋白的作用区域及与核基质的相互关系。材料与方法 :用免疫印迹法检测急性髓系细胞白血病外周血白细胞中Stat1蛋白的表达 ;结果 :在41例急性髓系细胞白血病外周血白细胞中 ,Stat1激活的阳性率为53.7 % ,正常对照15例阳性率为0 %。激活的Stat1蛋白转移到细胞核内并与核基质结合 ;结论 :Stat1蛋白在急性髓系细胞白血病外周血白细胞中有异常激活 ,并与核基质结合 。

BACKGROUND&AIM: To learn the expression and activation of Stat1protein in the peripheral blood white cells of acute myeloid leukemia(AML)by combing to the nuclear matrix. MATERIAL AND METHODS: The Stat1activation were detected by western blot with anti_Stat1antibody. RESULT: The results showed that Stat1was actiˉvated in the22/41(53.7%)with AML and0%in the15normal controls,and activated Stat1existed in the nuclear matrix components. CONCLUSION: The conclusion denotes that over expression and activation of Stat1maybe play an important role in the process of AML by attaching to nuclear matrix.