蛇床种内变异的RAPD分析

Analysis on Intraspecific Variation of Cnidium monnieri by RAPD

目的 :从DNA分子水平上探讨不同地区产蛇床的种内变异。方法 :用随机扩增多态性DNA(RAPD)技术分析 9个产地的蛇床基因组DNA多态性 ,并构建树状聚类图。结果 :从 2 0个 10bp随机引物中筛选出 12个有效引物 ,共扩增出 75条DNA带 ,其中 6 4条表现出多态性。结论 :地理分布距离越小 ,蛇床的遗传差异越小 ,反之越大 ,但生态环境对蛇床遗传变异的作用也不可忽视。

Objective:To study the genetic variation of Cnidium monnieri from different regions.Methods:Random amplified polymophic DNA technique was used to analyze genetic polymorphy of Cnidium monnieri from 9 different regions, and dendrogram was constructed by UPGMA.Results:20 random primers were used for polymorphic selection. A total of 75 bands were amplified,64 of which were polymorphism.Conclusion:The smaller the geographic distances between two Cnidium monnieri,the smaller genetic differences.However,it should not be overlooked that natural environment,especially the local ecological conditions may also show some influences on the genetic variations among different C.monnieri samples.