益智胶囊对大鼠脑缺血再灌注后海马CA1区神经元迟发性死亡及学习记忆功能的影响

Effects of Capsule Yizhi on the Delayed Neuronal Death in Hippocampal CA1 Region and Memory Function after Global Cerebral Ischemia in Rats

目的 :观察益智胶囊对全脑缺血后大鼠海马CA1区神经元迟发性死亡及学习功能的影响。方法 :使用四血管闭塞 ( 4 VO)法制成大鼠急性全脑缺血再灌注模型。采用免疫组织化学方法计数脑缺血再灌注脑损伤后 1、2、4、8及 4 0d时 ,大鼠海马CA1区正常神经元数量 ;并用Morriss水迷宫观察脑缺血再灌注脑损伤后 4 0d时各组大鼠的学习记忆功能。结果 :从缺血再灌注后第 2d起 ,益智胶囊 ( 10 0mg/kg)组大鼠海马CA1区正常神经元数量明显多于缺血对照组 (P <0 0 1)。同时 ,MORRIS水迷宫法检测大鼠全脑缺血再灌注脑损伤后 4 0d时的学习记忆功能 ,益智胶囊 ( 10 0mg/kg)组大鼠明显好于缺血对照组大鼠 (P <0 0 1)。结论 :益智胶囊对全脑缺血后大鼠海马CA1区神经元具有保护作用 。

Objective:To observe the effects of Capsule Yizhi on the delayed neuronal death in hippocampal CA1 region and memory function after global cerebral ischemia in rats.Methods:The model of acute reperfusion injury after cerebral ischemia in rats was made by vertebral and carotid arteries occlusion. 1,2,4,8 and 40 days later,the neurons in hippocampal CA1 region were immunohistochemically stained and calculated.And the Morris water maze test was carried out to observe the learning and memory capacities in the 40th day.Results:The number of normal neurons was significantly higher in Capsule Yizhi(100mg/kg) group than that in the control group. Compared with the control group, Capsule Yizhi (100mg/kg) could significantly shorten time in finding the platform under the water surface in Morris water maze test.Conclusion:Capsule Yizhi had protective effects on neurons in hippocampal CA1 region,and presented promising effects on learning and memory dysfunction after global ischemia/reperfusion injury.