肤康片防治尿道吻合术后瘢痕狭窄的实验研究

Experimental Study on Preventing Postoperative Posterior Urethra Scar in Response to Tablet Fu Kang

目的 :研究肤康片防治尿道吻合术后尿道瘢痕狭窄的作用。方法 :新西兰兔 2 0只 ,制成尿道断裂模型后行尿道吻合。实验组 10只 ,灌胃给予肤康片 ;对照组 10只 ,灌胃给予生理盐水。三周后取尿道吻合口组织 ,组织切片用Masson染色 ,输入全自动图像分析系统 ,观察胶原纤维的面积。结果 :对照组尿道吻合口瘢痕组织中胶原纤维含量多、密集、排列紊乱 ,而实验组胶原纤维含量少、稀疏、排列较规则 ;每三个高倍视野胶原纤维的面积 ,实验组为 117 11± 10 17μm2 ,对照组为 136 4 3± 15 85 μm2 ,有显著性差异 (P <0 0 5 )。结论 :肤康片在 (兔 )尿道平滑肌的创伤愈合过程中有明显的抑制成纤维细胞增殖。

Objective:To find an effective measurement to prevent and decrease the posterior urethral scar stricture.Methods:Twenty New Zealand rabbits were chosen.All the rabbits were made posterior urethra disruption model,and at the same time,the urethra anastomosis operation was performed.Twenty rabbits were divided into two groups after operation at random. One group was treated by Tablet Fu Kang(experimental group),another group was treated by NS (control group).All the rabbits were killed three weeks later,the urethra anastomotic stoma tissue was chosen and stained with Masson.All the figures of slides were inputted into figure analytic system.Results:There were little collagenous fiber,sparsely and arranged in good order in scar tissue of urethra anastomotic stoma in experimental group. The area of collagenous fibers was 117.11±10.17 μm 2 in three visual fields.There were many collagenous fibers,densely,and arranged in disorder in scar tissue of urethra anastomotic stoma in control group,the relative area was 136.43±15.85μm 2.There was statistic significance when comparing the area of collagenous fiber in two groups (P<0.05).Conclusions:Tablet Fu Kang could restrain the hyperplasia of fibroblast in the process of wound healing of rabbit smooth muscle in urethra.