甘蓝型杂交油菜“蜀杂9号”种子纯度的RAPD鉴定

Identfication of Purity of the Hybrid Seed of Shuza No.9 (Brassica napus L.) with Random Amplified Polymorphic DNA Markers

通过 5 0个随机引物对“蜀杂 9号”亲本进行RAPD扩增 ,选出了两个能在父、母本间产生多态性扩增的随机引物S1 8和S31 ,在杂种F1 代中验证了它们的特异性和稳定性 ,证明随机引物S1 8和S31能分别在F1 代中稳定扩增出父本和母本的特异标记片段S1 8750 与S31 550 对该引物的RAPD PCR的反应体系优化后 ,对“蜀杂 9号”父、母本和杂种F1 代的单株进行了随机检测 ,结果表明这两个RAPD标记配合使用能稳定可靠地鉴定甘蓝型杂交油菜“蜀杂 9号”种子的纯度 .

Both maternal-specific and paternal-specific DNA bands were amplified with the DNA of parents of hybrid rapeseed 'Shuza No.9' by primer S18 and S31 which were selected from 10mer 50 RAPD primers analyzed in this study. Then the primers were used to amplify all the DNA of F_1 rapeseed and the results indicated that the specificity and stability of primer S18 and S31 was trusty.The hybrid seed purity of 'Shuza No.9' could be determined reliably with them.