肾性高血压大鼠主动脉对血管活性物质反应的性别差异

Gender differences of response of aorta to vaso-active substance in rats with renal hypertension

目的 :探讨肾性高血压大鼠主动脉对血管活性物质反应的性别差异。方法 :雄性与雌性Sprague Dawley大鼠 ,用二肾一夹 (2K1C)方法复制肾性高血压模型。术后随机分 4组 :雄性假手术、雄性 2K1C、雌性假手术、雌性 2K1C组 ,4w后测定血压和左心室重 /体重 ,并取胸主动脉进行离体血管环实验。结果 :术后 4w ,与假手术组比较 ,2K1C组平均动脉压显著升高 ,左心室重 /体重比显著增大 ,主动脉对乙酰胆碱 (Ach)的舒张反应明显减弱 ,对NE和高钾溶液的收缩反应明显增强。在假手术组 ,雄性与雌性大鼠术后 4w上述各项指标改变差异无显著性。在 2K1C组 ,与雌性大鼠相比较 ,雄性大鼠的改变更为明显。结论 :2K1C肾性高血压的发展过程中 ,主动脉对血管活性物质的反应存在性别差异。

Objective:To explore the gender differences of the response of aorta to vaso-active substance in rats with renal hypertension.Methods:Animals were divided into 4 groups: the male-sham, male-2K1C (two-kidney and one-clip),female-sham, and male-2K1C group. Four weeks after operation,arterial pressure and the left ventricular weight/body weight were measured and and experiment of the isolated aortic ring cut from thoracic aorta were performed.Results:Four weeks after operation,the mean arterial pressure compared with the sham groups,was significantly elevated and in ratio of the left ventricular weight/body weight was remarkably icreased,the diastolic response of aorta to Ach was signifecantly decreased and the systolic response of aorta to non-epinephrine and high-KCl solution remarkably elevated.In the sham group,there was no significant difference on the indices mentioned-above in the male and female rats after four weeks of operation.In 2K1C group,changes of the male rats,compared with the female one,were more significant.Conclusion:In the developing process of renal hypertension with 2K1C method,there are gender differences of the aorta vaso-active substances in Sprague-Dawley rats.