黄连解毒汤对AD大鼠的治疗作用及对细胞因子含量的影响

The effect of Huanglian Jiedu Tang on Alzheimer's disease and its influence on cytokines

目的 :探讨黄连解毒汤对老年性痴呆 (AD)的治疗作用及对细胞因子含量的影响。方法 :以 β 淀粉蛋白(Aβ)注射形成AD大鼠模型 ,以水迷宫实验进行学习记忆能力测试 ;以ELISA法测定细胞因子 :肿瘤坏死因子 (TNF α) ,干扰素 γ(INF γ) ,白介素 2 (IL 2 )变化。结果 :模型组学习记忆能力下降 ,逃避的平均潜伏期延长 ,进入盲端的错误次数增多 ;黄连解毒汤治疗后 ,学习记忆能力改善 ,P <0 .0 1;模型组TNF α ,INF γ ,IL 2含量水平较正常组增高 ,P <0 .0 1,黄连解毒汤治疗Ⅰ ,Ⅱ组则明显降低 ,P <0 .0 1。结论 :黄连解毒汤可能通过调整AD大鼠的免疫功能和增加抗炎而提高其学习记忆能力。

Objective: To investigate the effect of Huanglian Jiedu Tang on Alzheimer's disease,and its influence on cytokines. Method: AD rats models were made by Aβ injection,and then water-maze tests were made to investigate the study and memory ability. TNF-α,INF-γ and IL-2 were examined by ELISA. After treatment with Huanglian Jiedu Tang,the rats were evaluated again. Result: After Aβ injection,the study and memory ability of the rats was decreased. By the treatment with Huanglian Jiedu Tang,it was improved (P<0.01);TNF-α,INF-γ and IL-2 levels of AD rats were higher than those in control group(P<0.01);after treatment the levels came down (P<0.01). Conclusion: Huanglian Jiedu Tang can improve the study and memory ability of AD rats by changing their immune state.