经济动物的空肠弯曲菌带菌调查和细菌分离培养研究

Investigation on the Carrier, Isolation and Incubation of Campylobacter jejuni in Economic Animals

本文对伊沙褐蛋鸡、乌骨鸡、锦鸡、蓝马鸡及银黑狐等动物的空肠弯曲菌带菌率进行了调查,其结果分别为66.19％、13.33％、20％、10％和6.19％。同对对微需氧培养方法和培养时间、TTC琼脂平板、蛋鸡采样时机、样品运送以及增菌培养等一系列影响检出率的因素进行了研究。结果认为该菌微需氧培养时间以48小时为宜;TTC琼脂平板不宜做该菌的初次分离培养;产蛋前采样要比产蛋后采样的检出率高,差异极显著(P<0.01);样品运送时间、Cary-Blair运送培养基以及增菌培养等对检出率影响不显著(P>0.05),但同一样品的重复分离培养,明显提高了该菌的检出率。

The carrier rates of Campylobacter jejuni among economic animals,such as ISA brown hen, the black bone chicken, Chrysolophus pictus,Crossoptilon auritum, Vulpes fulvua and Ailurus fulgens, were investigated. The results showed that the carrier rates were 66.19%、13. 33%、20%、10% and 6. 19% respectively. The effects of some factors affecting the detection rates, such as method of incubating C. jejuni in candle jar. incubation time, TTC agar plate, sampling time from hens, specimens conveyance and the enrichment culture were also studied. The results were as follows; The suitable incubation time at 42℃～43℃ in candle jar was 48h. TTC agar plate was unsuitable to the primary isolation of C. jejuni. The detection rate from specimens sampled before laying was significantly higher than that after laying (P<0.01). The transport time, the Cary-Blair conveyance medium used and the enrichment culture had no obvious effect on the rate (P>0.05). The detection rate could be obviously raised, when repeated isolation was made.