摘要
目的 :筛选无毒棉籽水提物有效部位 (CTN)中抗抑郁活性成分。方法 :提取大脑皮质突触膜 ,以CTN或其中 5个黄酮类单体成分 (分别为CTN 4 ,CTN 7,CTN 8,CTN 9和CTN 10 )与突触膜共孵 ,放射免疫法测定腺苷酸环化酶 (adenylatecyclase ,AC)活性。结果 :与阳性药丁螺环酮 (5 HT1A受体部分激动剂 )类似 ,CTN及CTN 4 ,7,8,9,10均可剂量依赖性激活突触膜AC ,其中CTN 8在 5个黄酮类单体中活性最强。结论 :黄酮类化合物槲皮素 3 O 芹菜糖基芦丁糖苷 (即CTN 8)可能是CTN中最强的抗抑郁活性成分之一 ,并且可能是 5 HT1A受体激动剂。
Objective: To screen the antidepressant components in aqueous extract of detoxified cottonseeds (CTN). Methods: The synaptic membrane of rat cerebral cortex was extracted and incubated with CTN or its five flavonoid compounds (CTN-4, 7, 8, 9, 10) respectively. The adenylyl cyclase (AC) activity was measured by radioimmunoassay. Results: Just like the positive drug buspirone, a 5-HT 1A receptor partial agonist, CTN and its five flavonoid compounds all activated AC in synaptic membrane in a dose-dependent manner, with compound CTN-8 being the most effective. Conclusion: The CTN-8, quercetin-3-O-apionyl- rutinoside, may be one of the most effective antidepressant components in CTN, and be a 5-HT 1A receptor agonist.
出处
《军事医学科学院院刊》
CSCD
北大核心
2004年第3期250-251,263,共3页
Bulletin of the Academy of Military Medical Sciences
基金
北京市自然科学基金资助项目 ( 70 42 0 5 2 )
关键词
棉籽
腺苷酸环化酶
抗抑郁剂
药物筛选
cottonseed
adenylate cyclase
antidepressants
drug screening
