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体外抗体亲和力成熟的几种策略 被引量：3

Several strategies of antibody affinity maturation in vitro
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摘要 抗体在疾病诊断、治疗和预防方面发挥着举足轻重的作用。随着抗体工程技术的不断发展 ,抗体已经成为生物制药领域中的热点。而抗体工程发展所面临的两大难点问题之一就是如何提高基因工程抗体的亲和力。本文综述了有关基因工程抗体体外抗体亲和力成熟方面的最新进展。 It is important for antibody to diagnose, treat and prevent diseases. In recent years, antibody engineering made more progress and became the hotspot in the field of biological pharmacy. In this review, the advance of antibody affinity maturation which is one of the important elements of antibody engineering is described.
作者 林周 黎燕 沈倍奋
机构地区 军事医学科学院基础医学研究所
出处 《军事医学科学院院刊》 CSCD 北大核心 2004年第3期292-294,共3页 Bulletin of the Academy of Military Medical Sciences
关键词 抗体工程 抗体亲和力成熟 antibody engineering antibody affinity maturation
分类号 R392.11 [医药卫生—免疫学]
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参考文献9

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  • 2Yang PL,Schultz PG.Mutational analysis of the affinity maturation of antibody 48G7[J].J Mol Biol,1999,294(5):1191-1201.
  • 3Yang WP,Green K,Pinz-Sweeney S,et al.CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range[J].J Mol Biol,1995,254(3):392-403.
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  • 6Park SG,Lee JS,Choi IH.Affinity maturation of natural antibody using a chain shuffling technique and the expression of recombinant antibodies in Escherichia coli[J].Biochem Biophys Res Commun,2000,275(2):553-557.
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同被引文献44

  • 1孙志伟,王双,杜威世,俞炜源.抗肝癌人源化单链抗体hscFv_(25)的体外亲和力成熟[J].世界华人消化杂志,2004,12(11):2568-2571. 被引量：2
  • 2胡晓林,张春明.基因工程抗体亲和力成熟的策略[J].免疫学杂志,2006,22(6):702-704. 被引量：8
  • 3Conroy PJ, Hearty S, Leonard P, et al. Antibody production, design and use for biosensor-based applications[J]. Seminars Cell Developm Biol, 2009, 20(1): 10-26.
  • 4Pande J, Szewczyk MM, Grover AK. Phage display: Concept, innovations, applications and future[J]. Biotechnol Advances, 2010, 28(6): 849--858.
  • 5Sheedy C, MacKenzie R, Hall JC. Isolation and affinity mat- uration of hapten- specific antibodies [J]. Biotechnology Advances, 2007,25(4): 333-352.
  • 6Rajpal A, Beyaz N, Haber L, et al. A general method for greatly improving the affinity of antibodies by using combi- natorial libraries[J]. Proc Natl Acad Sci USA, 2005,102(24): 8466-8471.
  • 7Rothe C, Urlinger S, Ltihning C, et al. The human combinatorial antibody library I-IuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies[J]. J Mol Biol, 2008, 376(4): 1182-1200.
  • 8Chowdhury PS, Pastan I. Improving antibody affinity by mimicking somatic hypermutation in vitro[J]. Nature, 1999, 17(6): 568-572.
  • 9McCall AM, Shahied L, Amoroso AR, et al. Increasing the affinity for tumor antigen enhances bispecific antibody cytotoxicity[J]. J Immunol, 2001, 166(10): 6112- 6117.
  • 10Barderas R, Desme J. Affinity maturation of antibodies assisted by in silico modeling[J]. Proc Natl Acad Sci, 2008, 105(26): 9029-9034.

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二级引证文献9

军事医学科学院院刊
2004年 第3期

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