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抑制性消减杂交技术及其应用研究进展 被引量:19

Advances in suppression subtractive hybridization(SSH) and its application
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摘要 抑制性消减杂交是结合抑制性PCR和消减杂交技术发展起来的一种高效分离差异表达基因的方法 ,具有特异性高、假阳性率低、操作简便、灵敏度高等特点。在正常和异常状态下基因的表达变化、差异基因的克隆和鉴定对新基因的认识和基因表达调控有着重要意义。SSH在心血管疾病。
作者 何鹏 江渝
出处 《国外医学(临床生物化学与检验学分册)》 2004年第4期356-358,共3页 Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine
基金 重庆市卫生局资助项目 (No :0 0 2 0 0 5)
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  • 2Sangrador-Vegas A,Lennington JB,Smith TJ.Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization.J Cytokine,2002,17(2): 66-70.
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  • 7Rho J,Altmann CR,Socci ND,et al.Gene expression profiling of osteoclast differentiation by combined suppression subtractive hybridization (SSH) and cDNA microarray analysis.DNA Cell Biol,2002,21(8): 541-549.
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