端粒酶反义寡聚脱氧核苷酸对人胃癌细胞的影响

Effect of telomerase antisense oligodeoxynucleotides on the growth of human gastric cancerous cell line

目的 :观察端粒酶反义寡聚脱氧核苷酸对人胃癌细胞株的作用。方法 :用端粒酶反义寡聚脱氧核苷酸与人胃癌细胞株共同温育一定时间后 ,观察细胞形态变化 ,检测细胞DNA含量的分布 ,测定端粒酶活性。结果 :端粒酶反义寡聚脱氧核苷酸能诱导人胃癌细胞凋亡 ,抑制端粒酶活性 ,在形态学上表现为细胞膜起泡、染色质固缩、核碎裂、凋亡小体形成 ;电泳呈凋亡特征性Ladder带 ;流式细胞仪分析显示 ,在G1期前出现亚 2倍体凋亡峰 ;端粒酶活性抑制。结论 :端粒酶反义寡聚脱氧核苷酸能诱导胃癌细胞凋亡 ,抑制端粒酶活性 ,抑制端合成 。

Objective To observe the effect of telomerase antisense oligodexynucleotides on human gastric cancerous cell line. Methods Human gastric cell line (SGC-7901) were cultured with 1～5 μmol/L telomerase antisense oligodexynucleotides for various duration. Cell activity,morphologic changes and cell-DNA distribution were observed. The apoptotic cells were measured with flow cytometer. Telomerase activity was detected by TRAP-ELISA. Results Gastric cancerous cells cultured with 5 μmol/L telomerase antisense oligodexynucleotides presented apoptotic features: (1) intace cell membrane,chromatin condensation,nucleic fragmentation and apoptotic body formation; (2) agarose electrophoresis showed marked DNA ladder;(3) flow cytometry analysis showed a sub-G_1 cell peak.Conclusion Telomerase antisense oligodeoxynucleotides may inhibit telomerase activity and act as a specific growth inhibitor to human gastric cancerous cell line. It may be a new target of treatment to human gastric cancer.