摘要
葡萄花药培养能产生体细胞胚。体细胞胚的诱导及其频率高低与材料基因型、培养基和培养条件有关。试验结果表明,对花药进行低温和药物处理不能明显地促进体细胞胚胎发生,但通过改进培养基成份,添加AgNO_3和用液-固培养均能促进葡萄花药胚性愈伤组织的产生。供试的14个基因型材料中仅有3个能产生体细胞胚,而体细胞胚仅部分能萌发成正常苗。
Grape somatic embryo could be produced by anther culture. The somatic embryo induction and induction frequency were related to genotypes, media and culture conditions. Sametic embryogenesis had not been enhanced by pretrcat of low temperature and certain chemicals, but the production of embryonic callus could be enhanced by modifying medium component, supplying AgNO_3 and using liquid-solid medium. Only 3 genotypes produced somatic embryos in 14 cultured genotypes. Part of the somatic embryos could regenerate into normal plantlets.
出处
《甘肃农业大学学报》
CAS
CSCD
1993年第3期247-252,共6页
Journal of Gansu Agricultural University
基金
甘肃省教委资助项目
关键词
葡萄
花药培养
愈伤组织
grape
anther culture
somatic embryogenesis
