摘要
为寻找与异种移植细胞性排斥反应相关的新基因 ,在抑制消减杂交 (SSH )基础上 ,对一个与人氧化应激反应 1(OSR1)基因同源的猪内皮细胞上调表达基因片段CS3作进一步研究。半定量RT PCR结果证实 ,在人PBMC作用后 ,该基因表达的确上调。应用快速cDNA末端扩增 (RACE )方法 ,获得了猪OSR1(pOSR1)的全长cDNA序列。pOSR1cDNA序列全长 4 333个碱基 ,开读框架长 15 90个碱基 ,编码 5 2 9个氨基酸。与人OSR1(hOSR1)相比 ,编码区核酸一致性为 92 8% ,氨基酸一致性为 95 5 %。GenBank登录号为AY2 7135 6。其功能研究正在进行中。
In order to find out the novel gene associated with the cell-mediated pig to human xenograft rejection,gene fragment CS3 associated with the up-regulated expression of the porcine endothelial cells and homologous with the human oxidative stress responsive 1 (OSR1) was further investigated on the basis of suppression subtractive hybridization (SSH). Results of semi-quantitative RT-PCR demonstrated that this gene was really up-regulated in the interactions of human PBMC and porcine endothelial cells. The full-length cDNA sequence of porcine OSR1 (pOSR1) with a length of 4 333 bp was obtained through the rapid amplification of cDNA ends (RACE) technique,and the open reading frame (ORF) with a length of 1 590 bp encoded 529 amino acid residues was also obtained. This gene showed 92.8% of nucleotide identity and 95.5% of amino acid identity with human OSR1. The GenBank accession number of this gene is AY271356 and the study on the function of this gene is still undergoing.
出处
《现代免疫学》
CAS
CSCD
北大核心
2004年第4期268-271,共4页
Current Immunology
基金
国家自然科学基金重大资助项目 ( 3 9993 43 0 )
关键词
异种移植
排斥反应
内皮细胞
快速cDNA末端扩增
OSR1
xenotransplantation
rejection
endothelial cell
rapid amplification of cDNA ends
oxidative-stress responsive 1