褪黑素对系统性红斑狼疮样小鼠的影响

Effects of Melatonin on the Alterations in the Systemic Lupus Erythermatosus-Like Murine Model Induced by Campylobacter Jejuni CJ-S_(131)

为了观察褪黑素 (MT )对CJ S13 1所致的SLE样小鼠改变的影响并探讨其作用机制。采用空肠弯曲菌CJ S13 1和CFA混合免疫动物 ,诱导SLE样小鼠改变。结果表明MT 0 0 1、 0 10、 1 0mg/kg/d× 2 8d ,ig能部分或完全拮抗血清抗ssDNA和组蛋白IgG型自身抗体水平的升高 ,抑制ConA及LPS诱导的淋巴细胞增殖反应的增强 ,降低动物尿蛋白水平 ,减轻肾组织病变程度。提示一定剂量的MT对SLE样小鼠改变具有一定的保护作用。

In the present study,the effects of melatonin on the alterations in the systemic lupus erythematosus-like murine model induced by Campylobacter jejuni CJ-S 131 were observed in order to investigate its mechanism of actions. The CJ-S 131 strain of bacteria was inoculated together with complete Freund's adjuvant (CFA) to induce the alterations in the systemic lupus erythematosus-like murine model. It was found that the levels of the anti-single stranded DNA (ssDNA) IgG and the histone autoantibody were increased in the sera of mice 4 weeks after inoculation,and the proliferative responses of splenocytes to ConA and LPS were also increased. Analysis of urine in the mice of model group all showed a “++” to “++++” proteinuria (1～10 g/L),and the histopathologic studies demonstrated glomerular lesions. Melatonin in dosages of 0.01,0.1 and 1.0 mg/kg could partially or completely block the elevated levels of the anti-ssDNA IgG,inhibited the increased proliferative responses of lymphocytes induced by ConA or LPS,reduced the level of proteinuria and decreased the degree of renal lesions of mice. These results indicate that melatonin has modulating effects on the alterations in the systemic lupus erythematosus-like murine model.