三氧化二砷对A549细胞增殖抑制作用及其机制

Anti-Proliferation Effect of Arsenic Trioxide on Lung Cancer A549 Cells and its Mechanism

[目的]探讨三氧化二砷对肺癌A549细胞的增殖抑制作用及其作用机制。[方法]以不同浓度三氧化二砷作用于体外培养的A549细胞,MTT法检测细胞生长抑制率,应用流式细胞仪及Hoechst33258荧光染色法观察细胞凋亡,TRAP鄄PCR鄄ELISA法检测细胞凋亡前后的端粒酶活性。[结果]三氧化二砷可显著降低A549细胞端粒酶活性,抑制细胞的生长,诱导细胞发生凋亡,并呈现出明显的量-效与时-效关系;在Hoechst染色图片上可见细胞染色质浓缩及细胞核碎裂等典型的凋亡改变。[结论]三氧化二砷能抑制A549细胞的生长并诱导细胞发生凋亡,降低细胞端粒酶活性可能是其重要作用机制之一。

To investigate the anti-proliferation effect of arsenic trioxide on lung cancer A549cells and its mechanism.A549cells were cultured in different concentrations of arsenic trioxide medium in vitro.The inhibitory ratio of the cells were measured by MTT assay.Apoptosis was observed by flow cytome-try(FCM)and Hoechst33258fluorescence staining.The activity of telomerase was detected by TRAP-PCR-ELISA before and after apoptosis occurred.Arsenic trioxide could decrease the telomerase activity,cause apoptosis and inhibit the growth of A549cells significantly.The suppression was both in time-depen-dence and dose-dependence.Morphological changes including condensation of celluar chromatin and frag-mentation of nuclear were found in Hoechst33258fluorescence staining.[Conclusion]Arsenic trioxide can in-hibit the growth of A549cells and induce apoptosis.Telomerase activity reduced in A549cells may be one of the important mechamisms.