负载宫颈癌肿瘤裂解物的树突状细胞诱导的淋巴细胞反应

Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte

目的 :利用树突状细胞 (dendriticcell,DC)递呈肿瘤抗原的生物学特性 ,观察DC负载宫颈肿瘤裂解物抗原后能否促进同种异体T淋巴细胞增殖 ,并且活化为细胞毒性T淋巴细胞 (cytotoxicTlymphocyte ,CTL)。 方法 :宫颈癌患者外周血单核细胞体外经粒 -巨噬细胞集落刺激因子 (GM -CSF)和白细胞介素 - 4 (IL - 4 )诱导产生DC ,肿瘤裂解物和TNF -α共同促其成熟 ,与异体T淋巴细胞混合共育 ,以MTT法测定T细胞的增殖程度。结果 :宫颈癌患者外周血来源DC无论负载肿瘤组织裂解物还是传代肿瘤细胞裂解物 ,均可促进异体T细胞大量增殖 ,二者间无显著差异 (P <0 .0 5 )。结论 :DC能递呈宫颈癌肿瘤抗原 ,诱导产生大量抗原特异性CTL 。

Objective:To make use of the characteristics of presenting and processing tumor antigen of dendritic cell to enhance generation of isotypical but xenogenous T lymphocyte.Methods:Autologous dendritic cells were obtained by culturing peripheral blood monocyte of patients with cervical cancer in the presence of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Dendritic cells were loaded with tumor cell lysate and co-cultured with heterogenous PBMCs from patients to induce generation of tumor specific cytotoxic T cell.The generation rate was measured by MTT.Results:Tumor lysate-loaded DC which was isolated from peripheral blood monocyte of patients with cervical cancer could induce the generation of isotypical but xenogenous T lymphcyte.There was no significant difference between tumor tissue lysate and tumor cell strain lysate(P<0.05).Conclusion:These results indicate that DC could process and present tumor lysate antigen to stimulate generation of specific immunotherapy in patients with cervical cancer.