羟氯喹增加胰岛素抵抗大鼠模型胰岛素敏感性的实验研究

Experimental study on hydroxychloroquine raised insulin sensitivity in insulin resistance rat models

目的 :观察羟氯喹对高脂饮食大鼠胰岛素敏感性的影响。方法 :高脂大鼠灌服羟氯喹后用正常葡萄糖钳夹试验测定胰岛素敏感性 (GIR6 0 - 12 0 ) ,并测量血糖、血脂和胰岛素等。结果 :(1)高脂喂养 4周 ,大鼠的空腹血糖、胰岛素、血脂、体重均显著增高 ,胰岛素敏感性下降。 (2 )服用羟氯喹、罗格列酮和二甲双胍 2周后 ,高脂大鼠的胰岛素敏感性显著改善 ,GIR6 0 -12 0值分别为 9.3± 1.2、11.6± 1.4、9.8± 1.3、mg·kg - 1min - 1,服用羟氯喹大鼠的血糖、血脂显著低于服安慰剂的高脂大鼠。结论 :(1)高脂喂养法成功地制备了胰岛素抵抗大鼠模型。 (2 )羟氯喹能增加胰岛素敏感性 ,其作用与二甲双胍相似 ,但不及罗格列酮 ;羟氯喹对血糖。

Objective:To observe the effects of hydroxychloroquine on insulin sensitivity in high-fat feeding rats.Methods:After administrating hydroxychloroquine,the glucose infusion rate (GIR) determined by euglycemic clamp was used to measure the insulin sensitivity,and determining fasting plasma glucose,lipid and insulin.Results:(1)In high-fat feeding rats,the GIR60-120 was lower and the fasting plasma glucose,insulin,lipid levels were higher than those in normal feeding rats.(2)Hydroxychloroquine,rosiglitazone and metformin increased insulin sensitivity (the value of GIR60-120 was 9.3±1.2,11.6±1.4 and 9.8±1.3 mg.kg/min,respectively) in insulin resistance (IR) models.Moreover,the fasting glucose and some plasma lipid indices were improved in the IR rats with hydroxychloroquine administration.Conclusion:It is indicated that hydroxychloroquine could raise insulin sensitivity of IR rats with high-fat feeding,which was similar to metfomin but lower than rosiglitazone.In addition,hydroxychloroquine also could improve the glucose and blood lipid of the IR rats in some extent.