兔眼前段碱烧伤后房水细胞因子和一氧化氮在眼内炎症反应中的作用

An experimental study on cytokine and nitric oxide content in rabbit aqueous humor after alkali burn

目的 :探讨兔眼前段碱烧伤后房水中肿瘤坏死因子 -α(tumornecrosisfactor-α,TNF -α)及一氧化氮 (NO)于眼内炎性反应严重程度之间的关系。方法 :将新西兰白兔随机分为正常对照组和碱烧伤组 ,并于术后 1、3、7、14、30天观察术后眼内炎症反应 ,包括房水细胞计数和分类 ,同时测定房水TNF -α、NO-2 /NO-3 含量。采用SPSS软件包对实验数据进行方差分析和 q检验。结果 :碱烧伤后不同时间房水中细胞总数、TNF -α水平及NO-2 /NO-3 含量均比正常对照组增高 ,单核巨噬细胞及TNF -α、NO-2 /NO-3 于 7～ 14天高峰。房水中细胞总数、TNF -α水平及NO-2 /NO-3 含量与眼内炎性反应严重程度变化规律基本一致 ,即炎性反应重时含量高。结论 :碱烧伤后眼内炎性反应的严重程度与房水细胞因子TNF

Objective:To investigate the relationship among inflammatory reaction and cytokine levels,nitric oxide(NO) content in aqueous humor after alkali burn.Methods:12 adult New Zealand rabbits were divided into two groups(6 rabbits each):normal control group and alkali burn experimental group.Aqueous humor samples were aspirated on the alkali burn 1,3,7,14 and 30 days,and the total number of white blood cells in the aqueous humor and their subset distribution as well as tumor necrosis factor-α (TNF-α) and NO^-_2/NO^-_3 were counted.The data were analyzed using analysis of variance of SPSS software.Results:There was a higher number of inflammatory cells and a higher level of TNF-α and NO^-_2/NO^-_3 in alkali burn eyes than that in the normal control eyes.The number of mononuclear phagocyte and the levels of TNF-α,the content of NO^-_2/NO^-_3 in aqueous humor were the highest in alkali burn group in 7～14 days.The change of TNF-α,NO^-_2/NO^-_3 and inflammatory reaction were basically similar,that is ,the more serious the reaction,the higher the levels of the content.Conclusion:The intraocular inflammation after alkali burn is closely related to the change in cytokine levels and NO content in aqueous humor.