超早期联合应用中药对缺血性脑卒中大鼠脑组织结构、含水量及酶活性的影响

Effects of combined traditional Chinese medicines on brain tissue structure, water content and enzyme activity in rats with ischemic cerebral infarction at supr a-early period

目的:观察联合应用中药脑髓康、参麦注射液、蝮蛇抗栓酶超早期治疗对脑梗死模型大鼠神经缺损体征和脑细胞受损的改善作用及对脑组织超氧化物歧化酶、丙二醛的影响。方法:实验分为7组:模型制作后超早期治疗组,模型制作后2,4,6,10h治疗组,模型对照组,正常对照组。用颈内动脉注射月桂酸钠方法复制大鼠急性脑梗死模型,脑髓康灌胃给药,参麦注射液、蝮蛇抗栓酶股静脉注射给药。结果:超早期治疗组和2h治疗组对改善脑水肿有明显效果,超早期治疗组脑组织含水量犤(77.35±1.34)%犦与模型对照组犤(80.16±1.89)%犦比较明显减少(P<0.01);2h治疗组脑组织含水量犤(77.63±1.24)%犦与模型对照组比较明显减少(P<0.05)。4,6,10h治疗组对改善脑水肿效果不明显(P>0.05)。超早期治疗组和模型制作后2,4,6,10h治疗组均能明显升高脑内超氧化物歧化酶水平,降低丙二醛水平,与模型对照组犤超氧化物歧化酶水平为(2.94±0.56)NU/mg,丙二醛水平为(4.58±0.39)nmol/L犦比较均有显著性差异(t=4.48～9.28,P均<0.01)。电镜显示超早期治疗组和模型制作后2h治疗组脑内超微结构比模型组有明显改善。结论:联合应用中药脑髓康、参麦注射液、蝮蛇抗栓酶超早期治疗可明显减轻缺血脑组织水肿,减轻过氧化反应导致的损伤,改善微血管、神经细胞。

AIM:To inspect the effects of combined traditional Chinese drugs of naosuikang recipe, shenmai injection and ahalysantinfarctasum on the symptoms of neurologi c defect, improvement of cellular injury,superoxide dismutase(SOD) and malondial dehyde(MDA) in rats with cerebral infarction. METHODS:Seventy SD rats were randomly divided into 7 groups:supra-early treat ment group,2,4,6 and 10 hour treated groups,model control group and normal contr ol group.Rat models with focal cerebral ischemia were induced by laurate sodium injection to carotid artery.Gastric perfusion of naosuikang recipe,venous inject ions of shenmai and ahalysantinfarctasum were given. RESULTS:Obvious improvement of cerebral edema was observed in the supra-early treatment group and 2-hour treatment group;Compared with the model control gro up[(80.16±1.89)%],the water content in the supra-early treatment group[(77.35 ±1.34)%,P< 0.01] and 2-hour treatment group[(77.63±1.24)%,P< 0.05] were dec reased obviously; while those in the 4,6 and 10-hour treatment groups were not obviously improved(P >0.05).In the supra-early treatment group,2,4,6 and 10-ho ur treatment groups,the SOD activities were increased obviously,while the MDA le vels were reduced remarkably,and there were significant differences as compared with those in the model control group[(2.94±0.56) NU/mg and(4.58±0.39) nmol/L] (t=4.48-9.28,P< 0.01).Electron microscope showed that morphologic damage of bra in ultrastructure was obviously alleviated in the supra-early treatment group a nd 2-hour treatment group as compared with that in the model group. CONCLUSION:Combined traditional Chinese medicines of naosuikang recipe,shenmai injection and ahalysantinfarctasum can obviously reduce cerebral edema,relieve peroxidation induced injury,improve the ultrastructures of microvessel,nerve cel l and glial cell and encephaledem,and recover the function of neurons.