救脑益智胶囊对D-半乳糖老化小鼠海马神经元β-淀粉样肽及其相关蛋白表达的影响(英文)

Effect of jiunao yizhi capsule on beta-amyloid and its related protein of hip pocampal neuron in D-galactose-induced aging murine

背景:D-半乳糖老化小鼠脑内存在的β-淀粉样肽(β-amyloid,Aβ)产生过多,是否与脑老化过程有关?目的:观察中药救脑益智胶囊对D-半乳糖老化小鼠海马神经元Aβ及其相关蛋白表达的影响。设计:随机对照的实验研究。地点和对象:实验地点:首都医科大学宣武医院神经生化室。昆明小鼠60只,无特定病原体动物环境(SPF)级,雄性,体质量26～28g,由中国药品生物制品检定所实验动物中心提供。方法:采用免疫组织化学染色和免疫印记方法,检测D-半乳糖老化小鼠模型海马神经元Aβ、β-分泌酶、内质网Aβ结合蛋白(endoplasmicreticulumamyloidβ-peptidebindingprotein,ERAB)和早老蛋白-1的表达水平,并观察中药救脑益智胶囊的保护作用。主要观察指标:Aβ、β-分泌酶、ERAB和早老蛋白-1免疫组织化学染色结果和凝胶转移电泳分析。结果:对照组小鼠海马CA1区神经元有少量的Aβ及其相关蛋白表达,而D-半乳糖老化小鼠海马CA1区神经元Aβ及其相关蛋白表达水平明显增加,与对照组比较差异有高度显著性意义(P<0.01),中药组上述各种蛋白的表达结果与对照组接近。结论:D-半乳糖老化小鼠海马神经元存在着Aβ及其相关蛋白表达的上调,中药救脑益智胶囊可使之恢复正常水平,对神经元具有一定的营养和保护作用。

BACKGROUND:Whether the excessive production of beta-amyloid(β- amyloid,Aβ ) in the brain of D-galactose-induced aging murine may relate to cerebral agin g process? OBJECTIVE:To investigate the effect of Chinese medicine jiunao yizhi capsule o n Aβ and it's related protein of hippocampal neurons in D-galactose-induced aging murine(DGAM). DESIGN:A randomized controlled study. SETTINGS and MATERIALS:Setting was at the Department of Neurobiochemistry of X uanwu Hospital affiliated to Capital University of Medical Sciences.Sixty male K unming murines (SPF grade) with a body mass from 26 g to 28 g were obtained from Experimental Animal Center of China Pharmaceutical Biological Products Analysis Institute. METHODS:Immunohistochemical staining and western blotting methods were used in the assays of the expressive levels of Aβ,beta-site APP-cleaving enzyme(BACE ),endoplasmic reticulum amyloid β-peptide binding protein(ERAB) and presenilin -1(PS-1) of D-galactose-induced aging murine. The protective effect of jiuna o yizhi capsule was observed. MAIN OUTCOME MEASURES:The results of immunohistochemical staining and gel elec trophoresis analysis of Aβ,BACE,ERAB and PS-1. RESULTS:There were little expression of Aβ and its related protein in the ne urons of hippocampal CA1 region in the murines in control group.The expression l evel of Aβ and its related protein in the neurons of hippocampal CA1 region in DGAM increased significantly compared with that of the control group(P< 0.01);h owever,the results of Chinese medicine group were close to that of control group . CONCLUSION:The expression of Aβ and its related protein in hippocampal neuro ns of DGAM increases,which can be recovered by the application of Chinese medici ne jiunao yizhi capsule that has certain nourishing and protective reactions in neurons.