生物医学工程材料β磷酸三钙生物陶瓷的体外降解性能研究

Degradation in vitro of beta calcuim phosphate bio ceramics

目的对磷酸钙生物陶瓷在体外环境下的降解作用的定量和定性分析,评价其降解性能和生物学安全性。方法实验选用一种可控性微结构多孔生物陶瓷的不规则形颗粒,参照ISO10993-14的方法,将5g材料浸泡在极限溶液和模拟溶液中,经120h后分别过滤并称重,运用ICP法分析其滤液。结果在极限溶液中材料浸泡后残留质量增加63.7mg,滤液中钙、磷、钠含量明显增加;而在模拟溶液中浸泡后材料残留质量减少7.6mg,滤液中钙、磷含量增加。结论β-TCP多孔生物陶瓷在体外能被降解,其降解成分主要为钙和磷,为进一步探讨该材料的体内生物降解性奠定基础。

AIM:To study the degradation performance of newly developed Calcium Phosphate Bioceramics in the limited solution and stimulant solution in vitro after identification and quantification of degradation products from bioceramics. METHODS:Five grams of calcium phosphate bioceramics that were controllable microstructure and full of ostia were soaked in limited solution and stimulant solution according to the method of ISO10993 14.After 120 hours,the limited solution and stimulant solution were filtered and weighed, and the filteration was analyzed with ICP method. RESULTS:The main degradation products from the solution were Ca,P,Na.The quantity of the residual from the limited solution increased 63.7 mg, while decreased 7.6 mg in stimulant solution.CONCLUTION:Beta tricalcium phosphate(TCP) bioceramic full of ostia can be degradated in vitro, and the main degradation products are Ca and P,which can establish a base for investigating the biodegradation of the material in vivo.