摘要
目的探讨血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)对脊髓损伤后脊髓神经元凋亡和c-fos基因的影响及保护机制。方法将85只同龄Wistar大鼠,采用Allens的weightdropping(WD)法挫伤大鼠T11节段脊髓,于术后0,15min,1,2,4,8,12,24h经蛛网膜下腔导管各注入VEGF溶液15μL(含VEGF20μg),并与生理盐水组和正常对照组作对照。采用原位末端标记法(TUNEL法)检测脊髓损伤前后发生凋亡的脊髓神经元。采用免疫组化和原位杂交方法检测脊髓损伤前后c-fos基因的变化。结果正常组脊髓前角中未发现凋亡细胞,生理盐水组于伤后2h始出现凋亡神经元。VEGF组与生理盐水组相比较,凋亡神经元明显减少(P<0.01,t=24.25)。c-fos基因表达在正常脊髓组织中呈弱阳性,在生理盐水组中表达明显增加,VEGF组c-fos基因表达比生理盐水组明显减少(P<0.01,t=12.03)。结论脊髓损伤后脊髓神经元凋亡增多和c-fos基因表达显著增多,VEGF能抑制脊髓损伤后脊髓神经元凋亡和c-fos基因表达,从而保护损伤的神经组织。
AIM:To explore the effect and protective mechanism of vascular endothelial growth factor(VEGF) on apoptosis and c fos gene expression in spinal neurons of rats after spinal cord injury (SCI).METHODS: T11 spinal cord segment of 85 Wistar rats were bruised with Allens' weight dropping method. The VEGFtreated group received 15 μL VEGF liquid through the canal of subarachnoid space (20 μg) at once, 15 minutes, 1, 2, 4, 8, 12 and 24 hours after operation, which was compared with saline group and normal control group.Apoptotic neurons were detected by TUNEL staining.The c fos mRNA levels of the rats were determined by in situ hybridization,and immunohistochemical methods.RESULTS: There were no apoptotic neurons in spinal anterior horn of the normal group.The apoptotic neurons were found 2 hours later in the saline group, which were significantly decreased in the VEGF group compared with the saline group(P< 0.01,t=24.25).The c fos gene expression showed weakly positive in normal spinal cord tissue,and showed significantly increased in saline group.c fos gene expression in VEGF group was significantly decreased in VEGF group compared with saline group(P< 0.01, t=12.03).CONCLUSION:Apoptotic neurons and c fos gene expression in rat spinal cord can be markedly enhanced after SCI,VEGF can protect the apoptosis of spinal neurons and c-fos gene expression thereby protecting the injured nervous tissue.
出处
《中国临床康复》
CSCD
2004年第23期4736-4737,i003,共3页
Chinese Journal of Clinical Rehabilitation
