人脑胶质瘤基质金属蛋白酶-9的表达与肿瘤血管形成的相关性及对其肿瘤防治的意义

Correlation between the expression of matrix metalloproteinase-9 and angiogenesis in glioma and its significance for prevention and cure of tumor

目的观察基质金属蛋白酶-9(MMP-9)在胶质瘤中的表达情况与微血管密度(MVD)的关系,探讨MMP-9对胶质瘤血管形成的作用和意义。方法采用免疫组化S-P法检测58例不同病理分级人脑胶质瘤及10例正常脑组织标本中MMP-9和CD34的表达,测定其阳性细胞数和阳性血管数。结果胶质瘤中MMP-9表达于肿瘤细胞胞浆和血管内皮细胞,少数强表达者肿瘤细胞核也见阳性表达;不同病理分级胶质瘤中MMP-9阳性表达率分别为Ⅰ级42.9%,Ⅱ级65.0%,Ⅲ级86.7%,Ⅳ级88.9%,其MMP-9表达显著高于正常脑组织(P<0.01)且与胶质瘤恶性程度呈正相关(rs=0.597,P<0.05);不同病理分级胶质瘤中MVD差异有显著性意义(H=47.865,P<0.05);MMP-9表达与MVD显著正相关(rs=0.897,P<0.01)。结论MVD和MMP-9的表达与胶质瘤恶性程度呈正相关,可作为临床判断胶质瘤恶性程度、侵袭性及预后的重要指标;MMP-9在胶质瘤的血管形成中起重要作用,可通过促进肿瘤血管形成加速肿瘤的进展。

AIM:To observe the relation between the expression of matrix metalloproteinase 9 (MMP 9) and microvessel density (MVD) in glioma in order to explore the role and the significance of MMP 9 on glioma angiogenesis.METHODS:The expression of MMP 9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma were detected by immunohistochemical streptavidin peroxidase(S P) technique. The positive cells of MMP 9 and the positive microvessels were examined under binocular light microscope. RESULTS:The positive expression of MMP 9 in glioma was localized in the cytoplast and vascular endothelial cells.The positive rates of MMP 9 in glioma of grade I,II,III and IV were 42.9%,65.0%,86.7%and 88.9%. The expression of MMP 9 was obvious higher than normal brain tissue(P< 0.01) and correlated with the glioma malignancy(rs=0.597,P< 0.05). The expression of MMP 9 was significantly correlated with MVD(H=47.865, P< 0.05). MVD was correlated with the glioma malignancy (rs=0.897,P< 0.01). CONCLUSION: The expression of MMP 9 and MVD are correlated with the glioma malignancy, which are helpful to judge the malignancy, invasion and prognosis.MMP 9 plays an important role in angiogenesis of glioma and accelerates glioma malignant development by promotion angiogenesis.