活血化瘀汤对骨折早期血管内皮细胞因子活性的影响

Effect of huoxue huayu tang on the expression of vascular endothelial growth factor during early fracture healing

目的探讨活血化瘀汤对骨折早期局部血管内皮生长因子(vascularen-dothelialgrowthfactor,VEGF)表达的影响。方法72只雄性SD大鼠建立闭合骨折髓内固定动物模型,分活血化瘀汤组、生理盐水组。分别在24,48,72h,5,7,14d取骨折区组织,通过RT-PCR方法进行半定量分析VEGF的表达。结果VEGF在前24h表达开始增强;实验组24,48,72hVEGF表达较生理盐水组弱(P>0.05),5,7,14d活血化瘀汤组表达较生理盐水组强(P<0.05);5,7dVEGF表达到峰值(P<0.01)。结论骨折早期活血化瘀汤促进VEGF表达增强。

AIM:To explore the effects of huoxue huayu tang on the expression of local vascular endothelial growth factors (VEGF) during early fracture healing. METHODS: The animal models of closed fracture with intramedullary fixation were established in seventy two male SD rats. The 72 rats were divided into herb group (huoxue huayu tang) and saline group. The tissues in fracture area were obtained at 24, 48, 72 hours, 7 and 14 days. Semi quantitative analysis was used to detect the expression of VEGF through RT PCR method. RESULTS: It increased significantly in VEGF expression at 24 hours. The expression of VEGF was weaker in experimental group compared with the saline group at 24, 48 and 72 hours (P >0.05), which was stronger in the herb group compared with the saline group at 5, 7 and 14 days (P< 0.05). The expression of VEGF reached to the peak at 5 and 7 days (P< 0.01). CONCLUSION: Huoxue huayu tang in early fracture can accelerate the expression of VEGF.