应用改良的环扣式传感器进行在体跟腱生物力学测试(英文)

Biomechanical testing with modified buckle transducer on Achilles tendon in vivo

背景在体肌腱的生物力学测试国内报道较少。目的探讨在体跟腱的生物力学行为。设计随机对照实验研究。地点和对象实验在武汉大学生物医学工程实验室完成,对象为新西兰大耳白兔45只,雌雄不限,体质量1.85～3.25kg(由武汉大学医学院动物室提供)。干预对环扣式传感器进行改良,选择45只兔随机分3组,正常左侧跟腱组,正常右侧跟腱组,失胫神经支配的左侧跟腱组,以改良环式传感器分别对3组的跟腱进行生物学测试。实验和结果由两位有经验的专家检测。主要观察指标跟腱张力曲线,张力波峰值,时间,冲量。结果家兔跑跳时的张力曲线主要有4种即Ⅰ,Ⅱ,Ⅲ,Ⅳ,失胫神经失配状态下的一侧跟腱的生物力学参数[F3=(8.82±3.75)N,冲量=(37.95±14.86)N·ms]明显低于常态[F3=(16.75±5.65)N,冲量=(110.20±62.96)N·ms,P<0.05]。结论改良环扣式传感器用于在体跟腱的生物力学测试具有可行、稳定、灵敏等优点。

BACKGROUND: Biomechanical measurement of Achilles tendon lacks domestically.OBJECTIVE: To investigate the biomechanics of Achilles tendon in vivo.DESIGN: A randomized controlled experimental study was conducted.SETTING and PARTICIPANTS:The experiment was conducted in the Laboratory of Biomedical Engineering,Wuhan University.Forty five New Zealand rabbits of either sex,weighing 1.85-3.25 kg,were used as the subjects(supplied by the Animal Room,Medical College,Wuhan University).INTERVENTION: Forty five rabbits were randomly divided into 3 groups with 15 in each group: group of normal left Achilles tendon(group A),group of normal right Achilles tendon(group B),and group of left Achilles tendon without innervation of tibial nerve(group C). Biomechanical test with improved buckle tranducer was conducted.The experiment was conducted and the results were analyzed by two experienced specialists. MAIN OUTCOME MEASURES:Tension curves and biomechanical parameters of Achilles tendon of rabbits in vivo.RESULTS: The tension time curve can be mainly classified into four types(Ⅰ,Ⅱ,Ⅲ,and Ⅳ).The biomechanical parameters of Achilles tendon under non innervation condition[F3=(8.82±3.75)N,impulse=(37.95±14.86)N·ms] were significantly lower than those under normal condition [F3=(16.75±5.65)N,impulse=(110.20±62.96)N·ms,all P< 0.05].CONCLUSION: Modified buckle transducer can be used for measuring tension of Achilles tendon in vivo and is feasible,stable and sensitive.