软肝颗粒抗免疫性肝纤维化作用机制研究

Mechanism of Ruangan Granule in Counteracting Immune Liver Fibrosis in Rats

[目的]探讨软肝颗粒抗肝纤维化、肝硬化形成的作用机理。[方法]以秋水仙碱为阳性对照组,观察软肝颗粒高、低剂量组对免疫性肝纤维化大鼠血清转化生长因子β1(TGF β1),羟脯氨酶(Hyp),Ⅰ、Ⅲ型胶原,肝功能及肝脏病理变化的影响。[结果]软肝颗粒和秋水仙碱均能降低牛血清白蛋白(BAS)肝纤维化大鼠血清丙氨酸氨基转移酶(ALT)、碱性磷酸酶(AKP)水平,提高血清总蛋白(TP)和白蛋白(ALB)水平,与模型组比较有非常显著性差异(P<0.01),其中高剂量组降ALT能力优于秋水仙碱组(P<0.05);软肝颗粒和秋水仙碱能降低血清Ⅰ、Ⅲ型胶原,TGF β1水平和肝组织羟脯氨酸含量,与模型组比较有非常显著性差异(均P<0.01),其中高剂量组作用分别优于低剂量组和秋水仙碱组(P<0.05或P<0.01),低剂量组与秋水仙碱组比较无显著性差异(P>0.05);肝组织病理改善高剂量组优于秋水仙碱组。[结论]软肝颗粒能改善肝组织病理变化,恢复肝功能,降低血清TGF β1和Ⅰ、Ⅲ型胶原水平及Hyp含量,抑制肝纤维化、肝硬化的形成。

[Objective] To study the mechanism of Ruangan Granule (RG) in counteracting the experimental liver fibrosis and cirrhosis in SD rats. [Methods] As compared with colchicines, effect of RG in small and large dosages on serum transforming growth factor β (TGF β1 ), hydroxyproline (Hyp), collagen Ⅰ and Ⅲ , hepatic function and hepatic pathology were observed in rats. [ Results ] Both RG and colchicines could decrease serum levels of alanine aminotransferase (ALT) and alkaline phosphatase (AKP) and increase serum levels of total protein and albumin in rats with liver fibrosis induced by bovine serum albumin (P < 0.01 as compared with the model group). The effect of large-dosage RG in decreasing ALT level was better than that of colchicines (P < 0.05). RG and colchicines could also reduce the serum levels of collagen Ⅰ and Ⅲ , TGF β1 and Hyp (P < 0.01 compared with the model group), the effect of large - dosage RG being better than that of small-dosage RG and colchicines (P < 0.05 or P < 0.01), and the difference between small-dosage RG and colchicines was insignificant (P > 0.05). Effect of large-dosage RG in improving hepatic pathology was better than that of colchicines. [Conclusion] RG has an inhibitory effect on the formation of liver fibrosis and cirrhosis. Its mechanism may be related to the improvement of hepatic function, decrease of serum TGF ft , collagen Ⅰ and Ⅲ and Hyp levels.