温度和离子对紫膜LB膜光循环的影响

THE EFFECT OF TEMPERATURE AND IONS ON PHOTOCYCLE OF PURPLE MEMBRANE LB FILM

用闪光动力学光谱仪测量了水平拉制的紫膜LB膜中菌紫质中间体M_(412)的衰减过程,观察了温度和离子对M_(412)衰减过程的影响。实验结果表明:在一定的温度范围内(10℃-60℃),随着温度的升高,M_(412)的衰减速率加快。对M_(412)s的衰减的抑制作用,La^(3+)在低浓度时就很明显,而K^+则在较高浓度时才表现出来,Ca^(2+)的影响不明显;La^(3+)对M_(412)f的衰减无明显影响,K^+和Ca^(2+)则稍微加快了其速率,pH的变化(H^+浓度)明显影响到M_(412)的衰减速率,尤其在高pH情况,M_(412)s的衰减比正常pH值时要慢一个数量级。

The decay rate of bacteriorhodopsin photocycle intermediate M412 in purple membrane LB film treated by ions and temperature was studied. The results showed that the decay rate turned fast with increaseing temperature. The decay of M412s was inhibited by La3+ at low concentration, but by K+ at higher concentration. The decay of M412f was accelerated by K+ and Ca2+ except La3+. The change of pH affected the decay rate of M412, When pH value reached 11, the decay rate of M412s was much slower than that of normal pH value.