Th1/Th2细胞因子mRNA的表达与心脏移植免疫耐受的关系

The relationship between Th1/Th2 cytokines mRNA expression and immune tolerance to cardiac allografts in rats

目的 探讨 Th1/Th2细胞因子信使核糖核酸 (m RNA)表达改变与心脏移植免疫耐受的关系。 方法 采用大鼠腹部心脏移植模型 ,将 30只大鼠随机分成对照组、排斥反应组、免疫耐受组 ,每组 10只。观察移植心脏存活时间 ,供心病理学改变 ,受者脾和心脏中 Th1/Th2细胞因子白细胞介素 2 (IL - 2 )、γ干扰素 (IFN-γ)、白细胞介素 4(IL- 4 )、白细胞介素 10 (IL- 10 ) m RNA表达水平。 结果 免疫耐受组供心存活时间为 85 .2 8± 7.4 8天 ,较排斥反应组的 7.33± 1.0 3天显著延长 (P<0 .0 1) ;排斥反应组供心见大量炎性细胞浸润 ,免疫耐受组供心仅见少量炎性细胞浸润 ;排斥反应组 Th1细胞因子 IL- 2、IFN- γ m RNA表达较对照组增强 ,免疫耐受组减弱 ;排斥反应组 Th2细胞因子IL- 4、IL- 10 m RNA表达较对照组减弱 ,免疫耐受组增强。 结论 Th1/Th2细胞因子的动态平衡在移植耐受中起重要作用 ,Th1向

Objective To study the relationship between Th1/Th2 cytokines messenger ribonucleic acid (mRNA) expression and immune tolerance to cardiac allografts in rats. Methods Male DA rat hearts were transplanted to male Lewis rats using Ono's model and randomly divided into three groups: control group, rejection group, and tolerance group (each group 10 rats). Mean survival time (MST), histological changes, mRNA expression level of Th1/Th2 cytokines interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-4(IL-4), interleukin-10(IL-10) were measured. Results MST (85.28±7.48 d) of heart allografts in tolerance group was significantly longer than that(7.33±1.03 d) in rejection group. Only a few inflammatory cells infiltrated in cardiac allografts in tolerance group. The mRNA expression of IL-2, IFN-γ (Th1 cytokines) in rejection group were much stronger than those in control group, and in tolerance group were much lower; mRNA expression of IL-4, IL-10 (Th2 cytokines) in rejection group were much lower than those in control group,and in tolerance group were much stronger than those in control group. Conclusions The dynamic equilibrium of Th1/Th2 cytokines is very important in immune tolerance. The deviation of Th1 to Th2 is one of the mechanisms in immune tolerance.