乌贼墨糖胺聚糖的制备与理化性质研究

Study on the preparation and properties of glycosaminoglycans from the ink of Sepiella maindroni de Rochebrune

本实验采用胰蛋白酶、链霉蛋白酶两次酶解,乙醇沉淀和DEAE-纤维素离子交换柱分离,从新鲜无针乌贼(Sepiella maindroni de Rochebrune)墨中提取得到乌贼墨糖胺聚糖(GAG);经醋酸纤维素薄膜电泳和聚丙烯酰胺凝胶电泳证明乌贼墨GAG为均一性多糖;紫外扫描无蛋白质的特征吸收峰;红外光谱分析,样品具有典型的GAG吸收峰;对该GAG酸水解产物的薄层色谱、纸色谱研究表明该GAG主要由氨基半乳糖、葡萄糖醛酸、岩藻糖3种单糖组成,含量分别为25.6％,29.2％,23.3％;硫酸根和蛋白质含量分别为8.2％,3.5％。

One kind of glycosaminoglycans (GAG) was extracted and purified from the ink of Sepiella maindroni de Rochebrune by using the procedures of trypsin and pronase digestion, ethanol precipitation and DEAE-cellulose inon-exchange chromatography. It was proved to be homogeneous by polyacrylamide gel and cellulose acetate membrane electrophoresis. No protein absorption was shown in UV spectrum, but typical absorption of GAG was shown in the IR spectrum. It was identified by paper and thin layer chromatography of the acid hydrolyzate that the GAG was composed of galactosamine, glucuronic acid and fucose. The percentage content of galactosamine, glucuronic acid, fucose, sulfate and protein in the GAG was 25. 6%, 29.2%, 23.3%, 8.2% and 3. 5%, respectively.