反相高效液相色谱法测定蛹虫草中虫草素的含量

Analysis of Cordycepin in Cordyceps Militaris(L. Fr)Link by High Performance Liquid Chromatography

建立了反相高效液相色谱法定量分析蛹虫草中虫草素(cordycepin)的方法。实验方法:色谱柱为Waters NOVAPAK C183.9mm×300mm,颗粒直径4μm;流动相为KH2PO4-K2HPO4缓冲液(0.01mol／L,PH=6.86)+1％四氢呋喃;流速为1.0mL／min,检测波长为260nm。在该实验条件下,虫草素的线性范围为3.23μg/mL-129.0μg／mL(r=0.9999),最小检出限为0.25ng。虫草素回收率平均值为97.5％,RSD为0.887％。该方法灵敏、快速、准确,适用于人工培植虫草中活性成分虫草素的检测。

In this paper,a HPLC method for determining the contents of cordycepin in cordyceps militaris ( L. Fr) Link was reported. Methods: The column was Waters NOVA - PAK C18 3,9mm×300mm; mobile phase was KH2PO4 - K2HPO4(0.01mol/L,PH =6. 86) + 1% tetrahydrofuran; flow rate 1.0mL/min; detection wavelength 260nm. Results:The linearity range was 3. 23 μg/mL ~ 129. 0μg/mL( r = 0. 9999) for cordycepin. The average recoveries of cordycepin were 97. 5% . The lower limit of detection was 0. 25ng ( SN = 3 ) for cordycepin. Conclusion: This method is easy rapid and accurate. It has been used to determine the cordycepin in cordyceps militaris (L. Fr)Link with satisfactory results.