培养条件对海洋真菌P32生长及其次生产物抗菌活性的影响

Effects of Culture Conditions on Cell Growth and metabolites Antifungal Activity of Marities Fungus P32

研究了培养基配方、培养条件和培养时间对海洋真菌P32菌株产生抗真菌活性物质的影响,以期获得高效价的活性次生产物。实验结果表明:采用改良的PDA液体培养基(葡萄糖马铃薯液体培养基+0.1％蛋白胨+2％葡萄糖),P32菌株在初始pH6.0,培养液装液量100ml／500ml,经28℃,165rpm旋转振荡培养72小时次生产物的生物活性最强,次生产物对黑曲霉具有抑制作用。

Culture conditions were investigated to obtain their effects on cell growth and metabolites antifungul activity of marine fungus P32, in the hope that a high - potency bioactive substances could be found. The results showed that the strongest antifungul activity of metabolites from P32 strains was obtained when it was cultured in the improved liquid medium based on PDA (liquid medium based on PDA, 2% glucose and 0.1% peptone) with initial pH 6.0 and the size of 100ml in 500ml Erlenmeyer flask at 165rpm and 28℃ for 72h.