Nafion-MV修饰电极丙酮酸氧化酶生物传感器测定GPT的研究

Pyruvate Oxidase Biosensor for the Determination of GPT Based on the Glassy Carbon Electrode Modified with Nafion and Methyl Viologen

用Nafion-甲基紫精修饰电极为基底,以牛血清白蛋白戊二醛为交联剂,将丙铜酸氧化酶固定在电极上,制成丙酮酸氧化酶生物传感器.用这种生物传感器测定人体血清中谷丙转氨酶(GPT)的活性,共存的尿酸、抗坏血酸等电活性物质不干扰测定.测定GPT的活性范围为0～110U/L,响应时间为50s.该传感器具有灵敏度高、抗干扰能力强和响应快等特点.

Pyruvate oxidase biosensor has been constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize pyruvate oxidase on glassy carbon electrode modified with Nafion and methyl viologen(MV). Glutamic pyruvic transaminase is determined by its effect on pyruvate production of reaction of the alanine with α-ketoglutarate. The linear range is 0 -110 U/L, and the response time is 50 s, and this biosensor has high selectivity and sensitivity.