四川猪圆环病毒Ⅱ型的检测

Detection of Porcine Circovirus Type Ⅱ in Sichuan Province

据文献资料设计一对引物,初步建立了检测猪圆环病毒II型(PCV-2)核酸片段的PCR方法。从可疑病料中扩增出了预期长度为473bp的DNA片段,用另一套引物证实了PCR产物的特异性。首次证实了四川地区猪群中存在PCV-2感染,为进一步研究打下了基础。

A specific PCR method was used to detect the nucleic acid fragment of PCV-2 in a hogpen. As expected, length of the DNA fragment was 473bp,so the infection of PCV-2 in Sichuan was first confirmed.