菊属野生种、栽培菊花及种间杂种的RAPD分析

RAPD analysis of wild species,cultivars and interspecific hybrids in Dendranthema

以菊属野生种、栽培菊花及种间杂种为试材 ,采用RAPD方法研究其亲缘关系。从 4 0条随机引物中筛选出 15条 ,对 2 3份试材进行扩增 ,共产生 15 3条带 ,平均每条引物扩增出 10 2条带 ,多态性条带占 77 1%。通过遗传相似性矩阵及UPGMA聚类分析 ,结合杂交结实性与幼胚拯救成苗率 ,认为 :父母本亲缘关系近的遗传距离较小 ,杂交亲和性好 ,结实率较高 ,幼胚拯救易成功 ;反之杂交亲和性差 ,难以结实 ,幼胚拯救成苗率低。根据以上数据结合前人研究结果 。

The relationship of 23 wild species,cultivars and interspecific hybrids in Dendranthema was studied by RAPD analysis.15 random primers were screened to detect polymorphism among the total materials used.Of the 153 bands produced,77 1% were polymorphic.Each primer amplified an average of 10 2 bands.The results from pairwise similarity and UPGMA cluster analysis,combining with the fecundity of hybridization and rate of plantlet of embryo rescue,suggested that when parents have closer relationship,the genetic distance was closer,the compatibility of hybridization was better,fecundity was higher and embryo rescue was easier.When parents have further relationship,the results were contrary.According to the RAPD analysis and previous research,the origin and evolution of Dendranthema morifolium was discussed.