杯伞发酵培养基的响应曲面法优化研究

Optimization of cultivation medium Clitocybe sp.AS 5.112 for the extracellular polysaccharide production and mycelial growth by response surface methodology

在原有的Plackett Burman设计与实验结果基础上 ,采用响应曲面法 (RSM )研究了影响杯伞 (Clitocybesp )AS5 112生长与发酵胞外多糖的培养基关键成分的最佳水平。实验结果表明 ,当葡萄糖、酵母膏、胰蛋白胨、NaNO3 和MgSO4分别为 14 0 0、 5 11、 2 0 7、 2 0 0和 1 2 8g·L-1时 ,胞外多糖最大预测值为 10 81 80 μg·mL-1(以发酵醪计 ) ;当上述自变量为 2 4 0 0、 3 2 3、 1 5 2、 2 36和 1 6 0 g·L-1时 ,菌丝最大生长量为 6 81mg·mL-1(以发酵醪计 )。欲同时获得最大量的胞外多糖和菌丝量 ,则葡萄糖、酵母膏、胰蛋白胨、NaNO3 和MgSO4须为 2 1 74、 4 33、 1 6 5、 2 4 5和1 5 0 g·L-1,在此条件下 ,每毫升发酵醪可同时获得 10 11 30 μg的胞外多糖和 6 6

Based on the results of a previous Plackett Burman design,the most important factors affecting the yield of extracellular polysaccharide and mycelial growth of Clitocybe sp AS 5 112 in submerged cultivation were further investigated by response surface methodology.Five factor,i e.glucose,yeast extract,tryptone,NaNO 3 and MgSO 4,five level full factorial central composite design was employed.By solving the quadratic regression model equation using appropriate statistic methods,the optimum concentrations were determined:glucose,yeast extract,tryptone,NaNO 3 and MgSO 4 were 14 00,5 11,2 07,2 00 and 1 28 g·L -1 ,respectively,the predicted yield of extracellular polysaccharide was 1 081 80 μg·mL -1 .When the above factors were 24 00,3 23,1 52,2 36 and 1 60 g·L -1 ,respectively,the maximum mycelial biomass was 6 81 mg·mL -1 .In order to obtain the maximum yield of extracellular polysaccharide and mycelial biomass at the same time,the optimal concentrations of glucose,yeast extract,tryptone,NaNO 3 and MgSO 4 would be 21 74,4 33,1 65,2 45 and 1 50 g·L -1 ,respectively.In this situation,1 011 30 g·mL -1 of extracellular polysaccharide and 6 66 mg·mL -1 of mycelial biomass were gained simultaneously.