摘要
对甘薯茎尖外植体的取材大小,不同取材季节,不同消毒处理及不同生育时期的试验结果表明,外植体的大小对其茎尖离体培养的成苗率及脱毒率有显著差异,尤其是取材的季节和不同生育时期,对甘薯茎尖培养的效果具有明显的差异,该试验以1～4月份取薯块催芽的外植体材料是甘薯茎尖培养较为适宜的培养时间,其污染、死亡率为15%;茎尖成活率85%以上;品种成苗率可达86%.
The Sweet potato(I pomoea batatas lam,) meristem culture and plantlet regeneration were studied, using different explants and different sterile methods in different growth stages.The results showed that the sizes of explants is important for plantlet rations and virus free rations, especially the season and the growth stage of materials. Were the feasible time to take the sweetpotato roots forFrom january to April accelerating germination, and the explants were more feasible for cristem culture. The rate of ecesis of cristem was more than 85% and variety plantlet ration could reach 86%.
出处
《中国农学通报》
CSCD
2004年第4期121-122,131,共3页
Chinese Agricultural Science Bulletin
基金
国家科技基础性工作专项资金项目
关键词
外植体
甘薯
茎尖培养
植株再生
培养基
Sweet potato
Explant
meristem culture
Plantlet regeneration
