摘要
测定血清中葡萄糖含量是临床检验的重要项目之一。何立千曾对各种血糖测定方法做过综述。葡萄糖氧化酶-4-氨基安替比林-苯酚光度法(酶制剂法)虽能较好地测定血清中葡萄糖的含量,但成本较高,耗时长。因此有必要研究一种快速、廉价、稳定的分析方法。人们考虑到酶的专属性,对葡萄糖氧化酶电极做了大量工作,然而,这种电极的寿命仍不理想。
An electrochemical detector cell for FIA of glucose in serum has been developed. It was a three electrodes system involved a homemade copper-PTFE composite plated film electrode (working) and other two electrodes, silver (reference) and platinum(auxiliary) wires. The carrier liquid for FIA was 0. 5 mol/L NaOH and flowed through the cell at 2.1 mL/min. The working electrode potential was set at 0. 6 V(vs. reference) by potentiostat. Sampling 30 ftL test solution which was 1% serum sample diluted with water, a peak current was recorded by chronome-try. The peak current was linearly related with the concentration of glucose in the range of 5 × 10-3-2×10-1 mmol/L. The analytical results from our method were pretty correlated to those from glucose oxidase-4-aminoanti-pyrine-phenol method(Trinder method). The regression equation from the results of twenty samples was Y=-0. 039 + 1. 005X, r=0. 9999. It was shown that our method was speedy, cheap and reliable.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1993年第11期1526-1527,共2页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
血清
葡萄糖
流动注射分析
Glucose in serum, Flow injection analysis, Electrochemical detector cell