利用双波长薄层扫描法测定多糖的单糖组成

Separation and determination of monosaccharides in polysaccharide by dual-wavelength thin-layer scanning technique

采用双波长薄层扫描法定量测定了一种茶叶多糖的单糖组成。结果表明,该方法对阿拉伯糖、葡萄糖、半乳糖、木糖的线性范围为1 200～7 200μg;4种单糖的回收率分别为97 6%、98 5%、98 1%、95 9%;所测定的这种茶多糖的单糖组成比例为阿拉伯糖∶葡萄糖∶半乳糖∶木糖=10 3∶11 4∶8 4∶1 3。实验证明其方法具有分离效果好,操作简便,速度快,实验成本低等优点。

A dual-wavelength thin-layer scanning technique for determination of monosaccharide mixture was presented in this paper.The experiment shows:the linear relationship of arabinose,D-glucose,D-galactose, D-xylose were good within range of 1.200-7.200μg.The recovery rates of the four monosaccharides were 97.6%,98.5%,98.1%,95.9% respectively.Monosacchride composition of the polysaccharide was arabinose ∶D-glucose ∶D-galactose ∶D-xylose=10.3 ∶(11.4 ∶8.4 ∶1.3.)The method is simple,quick,accurate and low-cost.