泥蚶同工酶谱在不同组织的差异研究

Studies on the tissual difference of isozyme patterns of Tegillarca granosa

用聚丙烯酰胺电泳法对泥蚶5种组织中的19种同工酶进行检测分析,发现除其中4种酶未显示任何有活性的区带,两种酶只显示极微弱且不稳定的区带外,其他种酶类在各个组织中都显示出清晰稳定的图谱,且这些酶在分布和活性上均表现出高度的组织特异性,说明泥蚶组织中已存在相当丰富和完整的酶系统,基本具备了与高等动物相类似的机体代谢方式,而且这些酶基因的表达存在着组织分布上的自我调控机制.同时与其他贝类和高等动物相比,泥蚶的同工酶基因的表达既表现出了与其分类地位相一致的特征,又反应出在分类阶元上的特殊性.

Nineteen isozymes in 5 tissues of Tegillarca granosa were tested by vertical polyacrylamide gel electrophoresis. The result is that :except 4 isozymes show no obvious bands and 2 isozymes show weak and unsteady bands, the rest isozymes present clear and steady bands in different tissues. It not only shows that Tegillarca granosa has possessed abound and complete isozyme system similar to that of advanced animals but also that certain self-regulation mechanism must exist in the expression of enzyme genes. Meanwhile, comparing with advanced animals and other shellfishes the enzyme genes expression of Tegillarca granosa shows both concordant and specific characteristics in line with its taxonomical position.