摘要
The multiplex polymerase chain reaction (PCR) technique was applied to detectthe SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNAfragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificiallyaccording to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of HongKong, and were used as simulated positive samples. Five primers recommended by World HealthOrganization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three targetcDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two ofthese fragments, were amplified by single PCR. The combination of these three fragments wasamplified by multiplex PCR. The results indicated that the multiplex PCR technique could be appliedto detect the SARS-CoV specific target cDNA fragments successfully.
The multiplex polymerase chain reaction (PCR) technique was applied to detectthe SARS-CoV (severe acute respiratory syndrome-associated coronavirus) specific target cDNAfragments in the present study. The target cDNA fragments of SARS-CoV were synthesized artificiallyaccording to the genome sequence of SARS-CoV in GenBank submitted by The Chinese University of HongKong, and were used as simulated positive samples. Five primers recommended by World HealthOrganization (WHO) were used to amplify the fragments by single PCR and multiplex PCR. Three targetcDNA fragments (121, 182 and 302 bp), as well as the three different combinations of any two ofthese fragments, were amplified by single PCR. The combination of these three fragments wasamplified by multiplex PCR. The results indicated that the multiplex PCR technique could be appliedto detect the SARS-CoV specific target cDNA fragments successfully.
基金
The Science Founda-tion of Fujian Entry-Exit Inspection and Quarantine Bureau (FK2003-35).
