甘草亲缘关系的RAPD鉴定

Relationship Analysis of Glycyrrhiza by RAPD

以不同地区的3种15组甘草(Glycyrrhizauralensis、G.infleta、G.glebra)种子为材料,探讨RAPD分子标记在研究、鉴定甘草亲缘关系中的可行性。从50个随机引物中筛选出9个有效引物,9个引物共扩增出961条DNA带,其中多态性条带811条,占总扩增条带的84.4%。对扩增出来的961条DNA带进行分析,结果表明:15组甘草植物的平均遗传距离为0.41,其中采自甘肃民勤野生甘草与采自新疆布尔津乌拉尔野生甘草遗传距离最小,为0.26;而采自内蒙古杭锦旗上海升袖的野生甘草与采自青海贵德的乌拉尔野生甘草遗传距离最大,为0.63。由RAPD聚类分析结果得出15组甘草植物之间的亲缘关系与形态学分类结果存在差异。

The phylogenetic relationships of three species of Glycyrrhiza (G.uralensis,G.infleta,G.glebra) were analyzed by RAPD.Nine random primers of high stable quality were screened from 50 primers.The cluster analysis results of 961 amplified DNA fragments were indicated as follows:the average genetic distance among the fifteen groups of Glycyrrhiza was 0.41,of which the max genetic distance was 0.63 while the min 0.26.The results show that there is some difference between RAPD cluster analysis and the original species.