多炔化合物氧化胁迫下稗草细胞保护酶系的反应

Response of Protective Enzyme in Callus of Barnyard Grass under Oxidizing Stress Induced by Polyacetylene Compound

用多炔类化合物1-苯基-4-(3,4-亚甲二氧)-苯基丁二炔(简称化合物5)处理稗草(Echinochloacrusgalli)愈伤组织,经紫外光(320～400nm)照射后,诱导细胞内形成氧化胁迫环境。利用生化酶学方法,测定几种保护酶系在氧化环境下的活性变化。发现经化合物5和照光处理后,可诱导激活细胞内的谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化物酶(POD)的活性,而超氧化歧化酶(SOD)则表现为活性受抑制。以0.1～10mg/L的浓度处理,所测GST、GSH-Px和POD的照光诱导活性明显高于未经照光处理的活性。其中以10mg/L的浓度处理,照光所提高3种酶活性的百分率分别为10.47%、113.68%和166.68%。以1mg/L和10mg/L的浓度处理,照光对SOD的抑制百分率分别为50.25%和76.46%。测定结果表明:在外源光敏物质引起细胞内的氧化胁迫环境下,可激活细胞内保护酶的活性,用于抵御氧化逆境对细胞的损伤。而SOD则可能是化合物5光活化抑制稗草生长的生化作用靶标酶之一。

The callus of barnyard grass (Echinochloa crusgalli)was treated with compound 5 (1-phenyl-4-(3,4-methylenedioxy)-phenyl-diacetylene) under UV-A irradiation for 3 h, which forms an inner environment of oxidizing stress in the cells. Exploiting the method of bioche-mistry, we assay the activity of several protective enzymes in the oxidizing stress. The results showed that compound 5 can stimulate the activity of glutathione-S-transferase(GST), glutathione peroxidase (GSH-Px) and peroxidase(POD) and inhibited the activity of superoxide dismutase (SOD) under UV-A. The activities of GST, GSH-Px, and POD under light were higher than those under non-light. The enhanced percentages of GST, GSH-Px, and POD at the concentration of 10 mg/L under light were 10.47%, 113.68% and 166.68%, but SOD was inhibited at 1 mg/L and 10 mg/L, the inhibiting percentage was 50.25% and 76.46%, respectively. The relationship between compound 5 oxidization and anti-oxidization was discussed in the paper.