丛枝菌根真菌培养方法研究进展

ADVANCE OF CULTURAL METHODS FOR ARBUSCULAR MYCORRHIZAL

文章结合最新研究成果着重从十个不同的层面对丛枝菌根真菌(AM真菌)的培养方法研究做了较为系统的介绍与评述。认为活体宿主植物盆栽培养法是最简单、最容易、也是最可靠的AM真菌培养方法,玻璃珠分室培养可方便地将培养基质与AM真菌分开,能获得纯净的AM真菌菌体,具有其它方法不可替代的作用,AM真菌单孢无菌的分室Ri T-DNA转型胡萝卜根双重离体培养是获得AM真菌纯净菌体和研究AM真菌遗传、生理、生化等特性的理想方法,以此方法为基础,在根室中补充碳源、在菌丝生长室置换培养基、多次收获菌体的转型根改良双重培养法是提高某些AM真菌孢子产量的力荐方法。

Review of culture methods for arbuscular mycorrhizal fungi (AMF) was made in ten different levels with the latest research achievement. The conclusion from which is as follows: open pot culture of living plants inoculated with AM fungi is the simplest, the easiest and the most available method for producing AM fungi propagates. Pure AM fungi spores and mycelium can be conveniently separated from the medium by the method of glass beads spilt-compartment culture, and it抯 important role can not be replaced by other methods. The dual monoaxenic culture of AM fungi with Ri T-DNA transformed carrot root in a split- compartment system is an ideal method for harvesting pure spores and mycelium of AM fungi without contaminants , and also a good way for the studies on the genetics , physiology and biochemisty of AM fungi. Depending on this basical technique, a new improved split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza is recommended which increased AM fungi spore by large quantities of spores.