期刊文献+

原核基因工程中以包涵体形式表达产物的中试分离纯化研究 被引量:3

Pilot Purification of Recombinant Proteins Expression in E. colias Inclusion Bodies
下载PDF
导出
出处 《高技术通讯》 EI CAS CSCD 1993年第9期11-15,共5页 Chinese High Technology Letters
关键词 蛋白 包涵体 分离 提纯 基因工程 Recombinant Protein Inclusion body Purification Pilot
  • 相关文献

同被引文献14

  • 1Misawa S, Kumagai I.Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies [J]. Biopolymers, 1999,51 : 297- 307.
  • 2Hlodan R, Craig S, Pain R H.Protein folding and its implications for the production of recombinant proteins [ J ].Biotech Genet Eng Rev, 1991,9: 47- 88.
  • 3De Bemardez Clark E.Protein refolding for industrial process [J].Current Opinion in Biotechnology, 2001,12: 202 -207.
  • 4Patra A K, Mukhopadhyay R,Mukhija R, et al.Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli [ J ] .Protein Expr Purif, 2000, 18: 182-192.
  • 5Guise A D, West S M, Chaudhuri J B.Protein folding in vivo and renaturation of recombinant proteins from inclusion bodies [ J ].Mol Biotech, 1996,6 ( 1 ):53-64.
  • 6Dabora J M, Sanyal G, Middaugh C R.Effect ofpolyanion on the refolding of human acidic fibroblast growth factor. [J]. J Biol Chem, 1991,266(35 ):23637-23640.
  • 7Builder S, Hart R, Lester P, et al.Refolding of misfolded insulin-like growth factor-Ⅰ [ J ]. US patent, 1997,663( 1 ): 304.
  • 8萨姆布鲁克J 拉塞尔DW 黄培堂译.分子克隆实验指南(第3版)[M].北京:科学出版社,2002..
  • 9张耀东,洪深求,张丽华,耿信笃.基因重组人Gamma-干扰素发酵和提取优化工艺的研究[J].陕西师大学报(自然科学版),1998,26(2):65-69. 被引量:7
  • 10滑静,杨佐君,门瑞霄.内源性阿片肽研究进展[J].北京农学院学报,1998,13(3):115-121. 被引量:7

引证文献3

二级引证文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部