体细胞克隆牛和转基因体细胞克隆牛的遗传学分析(英文)

Genetic Analysis of Cattle Cloned From Somatic Cells and Gene Transferred Somatic Cells

分析了来自同一细胞系的体细胞克隆牛甜甜、庆庆、浒娃及来源同一培养转基因体细胞系转基因体细胞克隆牛九妹、乐娃和 1个妊娠 8个月转基因流产胎牛 8C2以及随机抽取的 1头鲁西黄牛 (LX)、 1头褐斯坦牛 (HS)在 2 4个微卫星位点标记牛的基因型 .结果表明 2 4个多态位点均表现出多态 ,等位基因数为 1～ 5个 ,平均为 3 17个 .根据网上公布的数据 ,按其最高频率计算 ,甜甜、庆庆、浒娃、九妹、乐娃、 8C2与培养细胞系、转基因细胞系间匹配概率为 1 17× 10 -3 6,根据本研究观察到的数据计算 ,匹配概率为 1 90× 10 -2 3 ;而与随机抽取的 1头鲁西黄牛及褐斯坦牛的基因型分别在 2 3和2

Three cloned cattle from the same somatic cell line (TT, QQ, XW) and three transferred cloned cattle from the same gone transferred cell line (JM, LW, 8C2), together with two randomly selected cattle, one LUXI (LX) and one Holstein (HS), were genetically analyzed using 24 fluorescent microsatellite markers. There were 1 similar to 5 alleles at 24 loci, the average being 3.17. Among TT, QQ, XW, JM, LW, 8C2, the somatic cell line and the gene transferred somatic cell line, the probabilities of matching ( P,,) between individuals were 1.17 x 10(-36) using allele frequencies obtained from public data on internet and 1.90 x 10(-23) by defining the allele frequencies at each locus as the reciprocal of the number of alleles at that locus. In addition, their genotypes differed from those of the randomly selected LX and HS at 23 and 20 marker loci respectively.